Handbook of Flow Cytometry Methods

Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step--by--step procedures for: aeo the preparation and handling of standards,reagents and specimens aeo laboratory analyses for immunophenotyping aeo measurement of DNA content and cell cycle analyses aeo determination of cell physiology and function aeo set up and operation of flow cytometers aeo computational and data analysis techniques aeo safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the usera s needs. Special features of Handbook of Flow Cytometry Methods include: aeo ample space throughout for personal annotations aeo suggestions of alternative procedures; trouble--shooting tips aeo extensive list of reagents and suppliers aeo comb--bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine.