Cellular Imaging Techniques for Neuroscience and Beyond

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc.§The first comprehensive volume on cellular imaging with a focus for its application in neuroscience§Concluding chapter compares the merits of various techniques§Full color throughout, maximizing users comprehension of the results obtainable via various methods§Features outstanding and truly international scholarship, with chapters written by leading experts in neuroscience and cell biology§Discusses cutting edge methods such as STED, PALM/STORM, nonlinear microscopy and more§