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This laboratory manual with detailed background information and practical protocols will be a useful guide for researchers engaged in all areas of parasitology. Most techniques described here are suitable for helminths as well as protozoa, but also included are protocols for particular parasite species. Parasite infections are very widespread. However, effective therapeuticals are not yet available. The analysis of the life cycle and parasite host interactions at the molecular level may help in the search of the "Achilles heel" of a parasite and thus promote the development of new therapeutic approaches. Parasite molecules such as surface antigens, excretory proteins or metabolic enzymes may serve as targets for new diagnostics tests, chemo- or immunotherapeutics or even vaccination.