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Buch Analysis and improvement of the in vitro transfection efficiency of plasmid-DNA encoding for equine IL-12 used for melanoma therapy in horses María Carolina Durán Graeff

Analysis and improvement of the in vitro transfection efficiency of plasmid-DNA encoding for equine IL-12 used for melanoma therapy in horses

Name: Analysis and improvement of the in vitro transfection efficiency of plasmid-DNA encoding for equine IL-12 used for melanoma therapy in horses
Brand: Cuvillier Verlag
SKU: 12828362
Price: 22.6 EUR
Availability: InStock
Author: María Carolina Durán Graeff
ISBN: 9783954042845

María Carolina Durán Graeff

Sprache

Englisch

Buch Broschur

Libristo-Code: 12828362

Verlag Cuvillier Verlag, November 2012

In horses studies targeting equine melanoma therapy using DNA encoding for cytokines as interleukin... Vollständige Beschreibung

Libristo-Code: 12828362

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In horses studies targeting equine melanoma therapy using DNA encoding for cytokines as interleukin 12 (IL-12) have shown some promising results. Nevertheless, complete remission after treatment has been only observed in few cases, possibly due to reduced therapy efficiency. Herein transfection efficiency and methodology-induced cytotoxicity were analysed after transfection with different nanoparticle-mediated and conventional approaches using eukaryotic DNA-expression-plasmids and mammalian cell lines. The addition of gold nanoparticles (AuNPs) to the transfection protocols significantly increased transfection efficiency when compared to a conventional FHD mediated transfection protocol, and cell vitality was mainly negatively affected by the addition of chemically generated AuNPs. To measure accurately equine IL-12 and IFN-gamma concentrations after therapy, several antibodies for cross reactivity against this equine cytokines were evaluated, establishing afterwards a bead-based Luminex assay. Additionally, considering the valuable characteristics of dendritic cells (DCs), their use in further equine melanoma studies could be beneficial. To improve the still poor generation efficiency in horses, a human CD14 monoclonal antibody and an automated magnetic activated cell sorting system was used, reaching 2-fold higher DC yields than in previous published outcomes.

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Informationen zum Buch

Vollständiger Name Analysis and improvement of the in vitro transfection efficiency of plasmid-DNA encoding for equine IL-12 used for melanoma therapy in horses

Autor María Carolina Durán Graeff

Sprache

Englisch

Einband Buch - Broschur

Datum der Veröffentlichung 2012

Anzahl der Seiten 146

EAN 9783954042845

ISBN 3954042843

Libristo-Code 12828362

Verlag Cuvillier Verlag

Gewicht 199

Abmessungen 148 x 210 x 8

Kategorie

Medizin > Tiermedizin

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